Biological warfare

A biological attack could conceivably result in large numbers of civilian casualties and cause severe disruption to economic and societal infrastructure.^[9]

A nation or group that can pose a credible threat of mass casualty has the ability to alter the terms under which other nations or groups interact with it. When indexed to weapon mass and cost of development and storage, biological weapons possess destructive potential and loss of life far in excess of nuclear, chemical or conventional weapons. Accordingly, biological agents are potentially useful as strategic deterrents, in addition to their utility as offensive weapons on the battlefield.^[10]

As a tactical weapon for military use, a significant problem with biological warfare is that it would take days to be effective, and therefore might not immediately stop an opposing force. Some biological agents (smallpox, pneumonic plague) have the capability of person-to-person transmission via aerosolized respiratory droplets. This feature can be undesirable, as the agent(s) may be transmitted by this mechanism to unintended populations, including neutral or even friendly forces. Worse still, such a weapon could "escape" the laboratory where it was developed, even if there was no intent to use it – for example by infecting a researcher who then transmits it to the outside world before realizing that they were infected. Several cases are known of researchers becoming infected and dying of Ebola,^[11][12] which they had been working with in the lab (though nobody else was infected in those cases) – while there is no evidence that their work was directed towards biological warfare, it demonstrates the potential for accidental infection even of careful researchers fully aware of the dangers. While containment of biological warfare is less of a concern for certain criminal or terrorist organizations, it remains a significant concern for the military and civilian populations of virtually all nations.

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Civilian casualties

Civilian casualties

Civilian casualties occur when civilians are killed or injured by non-civilians, mostly law enforcement officers, military personnel, rebel group forces, or terrorists. Under the law of war, it refers to civilians who perish or suffer wounds as a result of wartime acts. The term is generally applied to situations in which violence is committed in pursuit of political goals. During periods of armed conflict, there are structures, actors, and processes at a number of levels that affect the likelihood of violence against civilians.

Smallpox

Smallpox

Smallpox was an infectious disease caused by variola virus which belongs to the genus Orthopoxvirus. The last naturally occurring case was diagnosed in October 1977, and the World Health Organization (WHO) certified the global eradication of the disease in 1980, making it the only human disease to be eradicated.

Pneumonic plague

Pneumonic plague

Pneumonic plague is a severe lung infection caused by the bacterium Yersinia pestis. Symptoms include fever, headache, shortness of breath, chest pain, and coughing. They typically start about three to seven days after exposure. It is one of three forms of plague, the other two being septicemic plague and bubonic plague.

Bioaerosol

Bioaerosol

Bioaerosols are a subcategory of particles released from terrestrial and marine ecosystems into the atmosphere. They consist of both living and non-living components, such as fungi, pollen, bacteria and viruses. Common sources of bioaerosols include soil, water, and sewage.

Ebola

Ebola

Ebola, also known as Ebola virus disease (EVD) and Ebola hemorrhagic fever (EHF), is a viral hemorrhagic fever in humans and other primates, caused by ebolaviruses. Symptoms typically start anywhere between two days and three weeks after becoming infected with the virus. The first symptoms are usually fever, sore throat, muscle pain, and headaches. These are usually followed by vomiting, diarrhoea, rash and decreased liver and kidney function, at which point, some people begin to bleed both internally and externally. The disease kills between 25% and 90% of those infected – about 50% on average. Death is often due to shock from fluid loss, and typically occurs between six and 16 days after the first symptoms appear. Early treatment of symptoms increases the survival rate considerably compared to late start. An Ebola vaccine was approved by the US FDA in December 2019.

Antiquity and Middle Ages

Rudimentary forms of biological warfare have been practiced since antiquity.^[13] The earliest documented incident of the intention to use biological weapons is recorded in Hittite texts of 1500–1200 BCE, in which victims of tularemia were driven into enemy lands, causing an epidemic.^[14] The Assyrians poisoned enemy wells with the fungus ergot, though with unknown results. Scythian archers dipped their arrows and Roman soldiers their swords into excrements and cadavers – victims were commonly infected by tetanus as result.^[15] In 1346, the bodies of Mongol warriors of the Golden Horde who had died of plague were thrown over the walls of the besieged Crimean city of Kaffa. Specialists disagree about whether this operation was responsible for the spread of the Black Death into Europe, Near East and North Africa, resulting in the deaths of approximately 25 million Europeans.^{[16][17][18][19]}

Biological agents were extensively used in many parts of Africa from the sixteenth century AD, most of the time in the form of poisoned arrows, or powder spread on the war front as well as poisoning of horses and water supply of the enemy forces.^[20][21] In Borgu, there were specific mixtures to kill, hypnotize, make the enemy bold, and to act as an antidote against the poison of the enemy as well. The creation of biologicals was reserved for a specific and professional class of medicine-men.^[21]

18th to 19th century

During the French and Indian War, in June 1763 a group of Native Americans laid siege to British-held Fort Pitt.^[22][23] The commander of Fort Pitt, Simeon Ecuyer, ordered his men to take smallpox-infested blankets from the infirmary and give it to a Lenape delegation during the siege.^[24][25][26] A reported outbreak that began the spring before left as many as one hundred Native Americans dead in Ohio Country from 1763 to 1764. It is not clear whether the smallpox was a result of the Fort Pitt incident or the virus was already present among the Delaware people as outbreaks happened on their own every dozen or so years^[27] and the delegates were met again later and seemingly had not contracted smallpox.^[28][29][30] During the American Revolutionary War, Continental Army officer George Washington mentioned to the Continental Congress that he had heard a rumor from a sailor that his opponent during the Siege of Boston, General William Howe, had deliberately sent civilians out of the city in the hopes of spreading the ongoing smallpox epidemic to American lines; Washington, remaining unconvinced, wrote that he "could hardly give credit to" the claim. Washington had already inoculated his soldiers, diminishing the effect of the epidemic.^[31][32] Some historians have claimed that a detachment of the Corps of Royal Marines stationed in New South Wales, Australia deliberately used smallpox there in 1789.^[33] Dr Seth Carus states: "Ultimately, we have a strong circumstantial case supporting the theory that someone deliberately introduced smallpox in the Aboriginal population."^[34][35]

World War I

By 1900 the germ theory and advances in bacteriology brought a new level of sophistication to the techniques for possible use of bio-agents in war. Biological sabotage in the form of anthrax and glanders was undertaken on behalf of the Imperial German government during World War I (1914–1918), with indifferent results.^[36] The Geneva Protocol of 1925 prohibited the use of chemical and biological weapons.^[37]

World War II

With the onset of World War II, the Ministry of Supply in the United Kingdom established a biological warfare program at Porton Down, headed by the microbiologist Paul Fildes. The research was championed by Winston Churchill and soon tularemia, anthrax, brucellosis, and botulism toxins had been effectively weaponized. In particular, Gruinard Island in Scotland, was contaminated with anthrax during a series of extensive tests for the next 56 years. Although the UK never offensively used the biological weapons it developed, its program was the first to successfully weaponize a variety of deadly pathogens and bring them into industrial production.^[38] Other nations, notably France and Japan, had begun their own biological weapons programs.^[39]

When the United States entered the war, Allied resources were pooled at the request of the British. The U.S. then established a large research program and industrial complex at Fort Detrick, Maryland in 1942 under the direction of George W. Merck.^[40] The biological and chemical weapons developed during that period were tested at the Dugway Proving Grounds in Utah. Soon there were facilities for the mass production of anthrax spores, brucellosis, and botulism toxins, although the war was over before these weapons could be of much operational use.^[41]

Shiro Ishii, commander of Unit 731, which performed human vivisections and other biological experimentation

The most notorious program of the period was run by the secret Imperial Japanese Army Unit 731 during the war, based at Pingfan in Manchuria and commanded by Lieutenant General Shirō Ishii. This biological warfare research unit conducted often fatal human experiments on prisoners, and produced biological weapons for combat use.^[42] Although the Japanese effort lacked the technological sophistication of the American or British programs, it far outstripped them in its widespread application and indiscriminate brutality. Biological weapons were used against Chinese soldiers and civilians in several military campaigns.^[43] In 1940, the Japanese Army Air Force bombed Ningbo with ceramic bombs full of fleas carrying the bubonic plague.^[44] Many of these operations were ineffective due to inefficient delivery systems,^[42] although up to 400,000 people may have died.^[45] During the Zhejiang-Jiangxi Campaign in 1942, around 1,700 Japanese troops died out of a total 10,000 Japanese soldiers who fell ill with disease when their own biological weapons attack rebounded on their own forces.^[46][47]

During the final months of World War II, Japan planned to use plague as a biological weapon against U.S. civilians in San Diego, California, during Operation Cherry Blossoms at Night. The plan was set to launch on 22 September 1945, but it was not executed because of Japan's surrender on 15 August 1945.^{[48][49][50][51]}

Cold War

In Britain, the 1950s saw the weaponization of plague, brucellosis, tularemia and later equine encephalomyelitis and vaccinia viruses, but the programme was unilaterally cancelled in 1956. The United States Army Biological Warfare Laboratories weaponized anthrax, tularemia, brucellosis, Q-fever and others.^[52]

In 1969, US President Richard Nixon decided to unilaterally terminate the offensive biological weapons program of the US, allowing only scientific research for defensive measures.^[53] This decision increased the momentum of the negotiations for a ban on biological warfare, which took place from 1969 to 1972 in the United Nation's Conference of the Committee on Disarmament in Geneva.^[54] These negotiations resulted in the Biological Weapons Convention, which was opened for signature on 10 April 1972 and entered into force on 26 March 1975 after its ratification by 22 states.^[54]

Despite being a party and depositary to the BWC, the Soviet Union continued and expanded its massive offensive biological weapons program, under the leadership of the allegedly civilian institution Biopreparat.^[55] The Soviet Union attracted international suspicion after the 1979 Sverdlovsk anthrax leak killed approximately 65 to 100 people.^[56]

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History of biological warfare

History of biological warfare

Before the 20th century, the use of biological agents took three major forms:Deliberate contamination of food and water with poisonous or contagious material Use of microbes, biological toxins, animals, or plants in a weapon system Use of biologically inoculated fabrics and persons

Hittite language

Hittite language

Hittite, also known as Nesite, is an extinct Indo-European language that was spoken by the Hittites, a people of Bronze Age Anatolia who created an empire centred on Hattusa, as well as parts of the northern Levant and Upper Mesopotamia. The language, now long extinct, is attested in cuneiform, in records dating from the 17th to the 13th centuries BCE, with isolated Hittite loanwords and numerous personal names appearing in an Old Assyrian context from as early as the 20th century BCE, making it the earliest-attested use of the Indo-European languages.

Ergot

Ergot

Ergot or ergot fungi refers to a group of fungi of the genus Claviceps.

Mongol Empire

Mongol Empire

The Mongol Empire of the 13th and 14th centuries was the largest contiguous land empire in history. Originating in present-day Mongolia in East Asia, the Mongol Empire at its height stretched from the Sea of Japan to parts of Eastern Europe, extending northward into parts of the Arctic; eastward and southward into parts of the Indian subcontinent, attempted invasions of Southeast Asia and conquered the Iranian Plateau; and westward as far as the Levant and the Carpathian Mountains.

Golden Horde

Golden Horde

The Golden Horde, self-designated as Ulug Ulus, lit. 'Great State' in Turkic, was originally a Mongol and later Turkicized khanate established in the 13th century and originating as the northwestern sector of the Mongol Empire. With the fragmentation of the Mongol Empire after 1259 it became a functionally separate khanate. It is also known as the Kipchak Khanate or as the Ulus of Jochi, and replaced the earlier, less organized Cuman–Kipchak confederation.

Black Death

Black Death

The Black Death was a bubonic plague pandemic occurring in Western Eurasia and North Africa from 1346 to 1353. It is the most fatal pandemic recorded in human history, causing the deaths of 75–200 million people, peaking in Europe from 1347 to 1351. Bubonic plague is caused by the bacterium Yersinia pestis spread by fleas, but during the Black Death it probably also took a secondary form, spread by person-to-person contact via aerosols causing pneumonic plague.

Hypnosis

Hypnosis

Hypnosis is a human condition involving focused attention, reduced peripheral awareness, and an enhanced capacity to respond to suggestion.

French and Indian War

French and Indian War

The French and Indian War (1754–1763) was a theater of the Seven Years' War, which pitted the North American colonies of the British Empire against those of the French, each side being supported by various Native American tribes. At the start of the war, the French colonies had a population of roughly 60,000 settlers, compared with 2 million in the British colonies. The outnumbered French particularly depended on their native allies.

Fort Pitt (Pennsylvania)

Fort Pitt (Pennsylvania)

Fort Pitt was a fort built by British forces between 1759 and 1761 during the French and Indian War at the confluence of the Monongahela and Allegheny rivers, where the Ohio River is formed in western Pennsylvania. It was near the site of Fort Duquesne, a French colonial fort built in 1754 as tensions increased between Great Britain and France in both Europe and North America. The French destroyed Fort Duquesne in 1758 when they retreated under British attack.

Lenape

Lenape

The Lenape also called the Lenni Lenape, and Delaware people, are an Indigenous people of the Northeastern Woodlands, who live in the United States and Canada.

American Revolutionary War

American Revolutionary War

The American Revolutionary War, also known as the Revolutionary War or American War of Independence, was the military conflict of the American Revolution in which American Patriot forces under George Washington's command defeated the British, establishing and securing the independence of the United States. Fighting began on April 19, 1775, at the Battles of Lexington and Concord. The war was formalized and intensified following passage of the Lee Resolution on July 2, 1776, which asserted that the Thirteen Colonies were "free and independent states", and the Declaration of Independence, drafted by the Committee of Five and written primarily by Thomas Jefferson, two days later, on July 4, 1776, by the Second Continental Congress in Philadelphia.

Continental Army

Continental Army

The Continental Army was the army of the United Colonies representing the Thirteen Colonies and later the United States during the American Revolutionary War. It was formed on June 14, 1775 by a resolution passed by the Second Continental Congress, meeting in Philadelphia after the war's outbreak. The Continental Army was created to coordinate military efforts of the colonies in the war against the British, who sought to maintain control over the American colonies. General George Washington was appointed commander-in-chief of the Continental Army and maintained this position throughout the war.

The Biological Weapons Convention^[57]

International restrictions on biological warfare began with the 1925 Geneva Protocol, which prohibits the use but not the possession or development of biological and chemical weapons.^[37][58] Upon ratification of the Geneva Protocol, several countries made reservations regarding its applicability and use in retaliation.^[59] Due to these reservations, it was in practice a "no-first-use" agreement only.^[60]

The 1972 Biological Weapons Convention (BWC) supplements the Geneva Protocol by prohibiting the development, production, acquisition, transfer, stockpiling and use of biological weapons.^[4] Having entered into force on 26 March 1975, the BWC was the first multilateral disarmament treaty to ban the production of an entire category of weapons of mass destruction.^[4] As of March 2021, 183 states have become party to the treaty.^[61] The BWC is considered to have established a strong global norm against biological weapons,^[62] which is reflected in the treaty's preamble, stating that the use of biological weapons would be "repugnant to the conscience of mankind".^[63] The BWC's effectiveness has been limited due to insufficient institutional support and the absence of any formal verification regime to monitor compliance.^[64]

In 1985, the Australia Group was established, a multilateral export control regime of 43 countries aiming to prevent the proliferation of chemical and biological weapons.^[65]

In 2004, the United Nations Security Council passed Resolution 1540, which obligates all UN Member States to develop and enforce appropriate legal and regulatory measures against the proliferation of chemical, biological, radiological, and nuclear weapons and their means of delivery, in particular, to prevent the spread of weapons of mass destruction to non-state actors.^[66]

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Geneva Protocol

Geneva Protocol

The Protocol for the Prohibition of the Use in War of Asphyxiating, Poisonous or other Gases, and of Bacteriological Methods of Warfare, usually called the Geneva Protocol, is a treaty prohibiting the use of chemical and biological weapons in international armed conflicts. It was signed at Geneva on 17 June 1925 and entered into force on 8 February 1928. It was registered in League of Nations Treaty Series on 7 September 1929. The Geneva Protocol is a protocol to the Convention for the Supervision of the International Trade in Arms and Ammunition and in Implements of War signed on the same date, and followed the Hague Conventions of 1899 and 1907.

Biological Weapons Convention

Biological Weapons Convention

The Biological Weapons Convention (BWC), or Biological and Toxin Weapons Convention (BTWC), is a disarmament treaty that effectively bans biological and toxin weapons by prohibiting their development, production, acquisition, transfer, stockpiling and use. The treaty's full name is the Convention on the Prohibition of the Development, Production and Stockpiling of Bacteriological (Biological) and Toxin Weapons and on their Destruction.

Reservation (law)

Reservation (law)

A reservation in international law is a caveat to a state's acceptance of a treaty. A reservation is defined by the 1969 Vienna Convention on the Law of Treaties (VCLT) as:a unilateral statement, however phrased or named, made by a State, when signing, ratifying, accepting, approving or acceding to a treaty, whereby it purports to exclude or to modify the legal effect of certain provisions of the treaty in their application to that State.

No first use

No first use

In nuclear ethics and deterrence theory, No first use (NFU) refers to a type of pledge or policy wherein a nuclear power formally refrains from the use of nuclear weapons or other weapons of mass destruction (WMDs) in warfare, except for as a second strike in retaliation to an attack by an enemy power using WMDs. Such a pledge would allow for a unique state of affairs in which a given nuclear power can be engaged in a conflict of conventional weaponry while it purposefully foregoes any of the strategic advantages of nuclear weapons, provided the enemy power does not possess or utilize any such weapons of their own. The concept is primarily invoked in reference to nuclear mutually assured destruction but has also been applied to chemical and biological warfare, as is the case of the official WMD policy of India.

List of parties to the Biological Weapons Convention

List of parties to the Biological Weapons Convention

The list of parties to the Biological Weapons Convention encompasses the states which have signed and ratified or acceded to the Biological Weapons Convention (BWC), a multilateral treaty outlawing biological weapons.

Australia Group

Australia Group

The Australia Group is a multilateral export control regime (MECR) and an informal group of countries established in 1985 to help member countries to identify those exports which need to be controlled so as not to contribute to the spread of chemical and biological weapons.

United Nations Security Council

United Nations Security Council

The United Nations Security Council (UNSC) is one of the six principal organs of the United Nations (UN) and is charged with ensuring international peace and security, recommending the admission of new UN members to the General Assembly, and approving any changes to the UN Charter. Its powers include establishing peacekeeping operations, enacting international sanctions, and authorizing military action. The UNSC is the only UN body with the authority to issue binding resolutions on member states.

United Nations Security Council Resolution 1540

United Nations Security Council Resolution 1540

United Nations Security Council resolution 1540 was adopted unanimously on 28 April 2004 regarding the non-proliferation of weapons of mass destruction. The resolution establishes the obligations under Chapter VII of the United Nations Charter for all member states to develop and enforce appropriate legal and regulatory measures against the proliferation of chemical, biological, radiological, and nuclear weapons and their means of delivery, in particular, to prevent the spread of weapons of mass destruction to non-state actors.

Chemical weapon

Chemical weapon

A chemical weapon (CW) is a specialized munition that uses chemicals formulated to inflict death or harm on humans. According to the Organisation for the Prohibition of Chemical Weapons (OPCW), this can be any chemical compound intended as a weapon "or its precursor that can cause death, injury, temporary incapacitation or sensory irritation through its chemical action. Munitions or other delivery devices designed to deliver chemical weapons, whether filled or unfilled, are also considered weapons themselves."

Nuclear weapon

Nuclear weapon

A nuclear weapon is an explosive device that derives its destructive force from nuclear reactions, either fission or a combination of fission and fusion reactions, producing a nuclear explosion. Both bomb types release large quantities of energy from relatively small amounts of matter.

Non-state actor

Non-state actor

A non-state actor (NSA) are organizations and/or individuals that are not affiliated with, directed by, or funded by any government.

Biological weapons are difficult to detect, economical and easy to use, making them appealing to terrorists. The cost of a biological weapon is estimated to be about 0.05 percent the cost of a conventional weapon in order to produce similar numbers of mass casualties per kilometer square.^[67] Moreover, their production is very easy as common technology can be used to produce biological warfare agents, like that used in production of vaccines, foods, spray devices, beverages and antibiotics. A major factor in biological warfare that attracts terrorists is that they can easily escape before the government agencies or secret agencies have even started their investigation. This is because the potential organism has an incubation period of 3 to 7 days, after which the results begin to appear, thereby giving terrorists a lead.

A technique called Clustered, Regularly Interspaced, Short Palindromic Repeat (CRISPR-Cas9) is now so cheap and widely available that scientists fear that amateurs will start experimenting with them. In this technique, a DNA sequence is cut off and replaced with a new sequence, e.g. one that codes for a particular protein, with the intent of modifying an organism's traits. Concerns have emerged regarding do-it-yourself biology research organizations due to their associated risk that a rogue amateur DIY researcher could attempt to develop dangerous bioweapons using genome editing technology.^[68]

In 2002, when CNN went through Al-Qaeda's (AQ's) experiments with crude poisons, they found out that AQ had begun planning ricin and cyanide attacks with the help of a loose association of terrorist cells.^[69] The associates had infiltrated many countries like Turkey, Italy, Spain, France and others. In 2015, to combat the threat of bioterrorism, a National Blueprint for Biodefense was issued by the Blue-Ribbon Study Panel on Biodefense.^[70] Also, 233 potential exposures of select biological agents outside of the primary barriers of the biocontainment in the US were described by the annual report of the Federal Select Agent Program.^[71]

Though a verification system can reduce bioterrorism, an employee, or a lone terrorist having adequate knowledge of a bio-technology company's facilities, can cause potential danger by utilizing, without proper oversight and supervision, that company's resources. Moreover, it has been found that about 95% of accidents that have occurred due to low security have been done by employees or those who had a security clearance.^[72]

Entomological warfare (EW) is a type of biological warfare that uses insects to attack the enemy. The concept has existed for centuries and research and development have continued into the modern era. EW has been used in battle by Japan and several other nations have developed and been accused of using an entomological warfare program. EW may employ insects in a direct attack or as vectors to deliver a biological agent, such as plague. Essentially, EW exists in three varieties. One type of EW involves infecting insects with a pathogen and then dispersing the insects over target areas.^[73] The insects then act as a vector, infecting any person or animal they might bite. Another type of EW is a direct insect attack against crops; the insect may not be infected with any pathogen but instead represents a threat to agriculture. The final method uses uninfected insects, such as bees or wasps, to directly attack the enemy.^[74]

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Entomological warfare

Entomological warfare

Entomological warfare (EW) is a type of biological warfare that uses insects to interrupt supply lines by damaging crops, or to directly harm enemy combatants and civilian populations. There have been several programs which have attempted to institute this methodology; however, there has been limited application of entomological warfare against military or civilian targets, Japan being the only state known to have verifiably implemented the method against another state, namely the Chinese during World War II. However, EW was used more widely in antiquity, in order to repel sieges or cause economic harm to states. Research into EW was conducted during both World War II and the Cold War by numerous states such as the Soviet Union, United States, Germany and Canada. There have also been suggestions that it could be implemented by non-state actors in a form of bioterrorism. Under the Biological and Toxic Weapons Convention of 1972, use of insects to administer agents or toxins for hostile purposes is deemed to be against international law.

Biological agent

Biological agent

A biological agent is a bacterium, virus, protozoan, parasite, fungus, or toxin that can be used purposefully as a weapon in bioterrorism or biological warfare (BW). In addition to these living or replicating pathogens, toxins and biotoxins are also included among the bio-agents. More than 1,200 different kinds of potentially weaponizable bio-agents have been described and studied to date.

Plague (disease)

Plague (disease)

Plague is an infectious disease caused by the bacterium Yersinia pestis. Symptoms include fever, weakness and headache. Usually this begins one to seven days after exposure. There are three forms of plague, each affecting a different part of the body and causing associated symptoms. Pneumonic plague infects the lungs, causing shortness of breath, coughing and chest pain; bubonic plague affects the lymph nodes, making them swell; and septicemic plague infects the blood and can cause tissues to turn black and die.

Pathogen

Pathogen

In biology, a pathogen in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a germ.

Theoretically, novel approaches in biotechnology, such as synthetic biology could be used in the future to design novel types of biological warfare agents.^{[75][76][77][78]}

1. Would demonstrate how to render a vaccine ineffective;
2. Would confer resistance to therapeutically useful antibiotics or antiviral agents;
3. Would enhance the virulence of a pathogen or render a nonpathogen virulent;
4. Would increase the transmissibility of a pathogen;
5. Would alter the host range of a pathogen;
6. Would enable the evasion of diagnostic/detection tools;
7. Would enable the weaponization of a biological agent or toxin.

Most of the biosecurity concerns in synthetic biology are focused on the role of DNA synthesis and the risk of producing genetic material of lethal viruses (e.g. 1918 Spanish flu, polio) in the lab.^[79][80][81] Recently, the CRISPR/Cas system has emerged as a promising technique for gene editing. It was hailed by The Washington Post as "the most important innovation in the synthetic biology space in nearly 30 years."^[82] While other methods take months or years to edit gene sequences, CRISPR speeds that time up to weeks.^[4] Due to its ease of use and accessibility, it has raised a number of ethical concerns, especially surrounding its use in the biohacking space.^[82][83][84]

Anti-personnel

The international biological hazard symbol

Ideal characteristics of a biological agent to be used as a weapon against humans are high infectivity, high virulence, non-availability of vaccines and availability of an effective and efficient delivery system. Stability of the weaponized agent (the ability of the agent to retain its infectivity and virulence after a prolonged period of storage) may also be desirable, particularly for military applications, and the ease of creating one is often considered. Control of the spread of the agent may be another desired characteristic.

The primary difficulty is not the production of the biological agent, as many biological agents used in weapons can be manufactured relatively quickly, cheaply and easily. Rather, it is the weaponization, storage, and delivery in an effective vehicle to a vulnerable target that pose significant problems.

For example, Bacillus anthracis is considered an effective agent for several reasons. First, it forms hardy spores, perfect for dispersal aerosols. Second, this organism is not considered transmissible from person to person, and thus rarely if ever causes secondary infections. A pulmonary anthrax infection starts with ordinary influenza-like symptoms and progresses to a lethal hemorrhagic mediastinitis within 3–7 days, with a fatality rate that is 90% or higher in untreated patients.^[85] Finally, friendly personnel and civilians can be protected with suitable antibiotics.

Agents considered for weaponization, or known to be weaponized, include bacteria such as Bacillus anthracis, Brucella spp., Burkholderia mallei, Burkholderia pseudomallei, Chlamydophila psittaci, Coxiella burnetii, Francisella tularensis, some of the Rickettsiaceae (especially Rickettsia prowazekii and Rickettsia rickettsii), Shigella spp., Vibrio cholerae, and Yersinia pestis. Many viral agents have been studied and/or weaponized, including some of the Bunyaviridae (especially Rift Valley fever virus), Ebolavirus, many of the Flaviviridae (especially Japanese encephalitis virus), Machupo virus, Coronaviruses, Marburg virus, Variola virus, and yellow fever virus. Fungal agents that have been studied include Coccidioides spp.^[55][86]

Toxins that can be used as weapons include ricin, staphylococcal enterotoxin B, botulinum toxin, saxitoxin, and many mycotoxins. These toxins and the organisms that produce them are sometimes referred to as select agents. In the United States, their possession, use, and transfer are regulated by the Centers for Disease Control and Prevention's Select Agent Program.

The former US biological warfare program categorized its weaponized anti-personnel bio-agents as either Lethal Agents (Bacillus anthracis, Francisella tularensis, Botulinum toxin) or Incapacitating Agents (Brucella suis, Coxiella burnetii, Venezuelan equine encephalitis virus, Staphylococcal enterotoxin B).

Anti-agriculture

Anti-crop/anti-vegetation/anti-fisheries

The United States developed an anti-crop capability during the Cold War that used plant diseases (bioherbicides, or mycoherbicides) for destroying enemy agriculture. Biological weapons also target fisheries as well as water-based vegetation. It was believed that the destruction of enemy agriculture on a strategic scale could thwart Sino-Soviet aggression in a general war. Diseases such as wheat blast and rice blast were weaponized in aerial spray tanks and cluster bombs for delivery to enemy watersheds in agricultural regions to initiate epiphytotic (epidemics among plants). On the other hand, some sources report that these agents were stockpiled but never weaponized.^[87] When the United States renounced its offensive biological warfare program in 1969 and 1970, the vast majority of its biological arsenal was composed of these plant diseases.^[88] Enterotoxins and Mycotoxins were not affected by Nixon's order.

Though herbicides are chemicals, they are often grouped with biological warfare and chemical warfare because they may work in a similar manner as biotoxins or bioregulators. The Army Biological Laboratory tested each agent and the Army's Technical Escort Unit was responsible for the transport of all chemical, biological, radiological (nuclear) materials.

Biological warfare can also specifically target plants to destroy crops or defoliate vegetation. The United States and Britain discovered plant growth regulators (i.e., herbicides) during the Second World War, which were then used by the UK in the counterinsurgency operations of the Malayan Emergency. Inspired by the use in Malaysia, the US military effort in the Vietnam War included a mass dispersal of a variety of herbicides, famously Agent Orange, with the aim of destroying farmland and defoliating forests used as cover by the Viet Cong.^[89] Sri Lanka deployed military defoliants in its prosecution of the Eelam War against Tamil insurgents.^[90]

Anti-livestock

During World War I, German saboteurs used anthrax and glanders to sicken cavalry horses in U.S. and France, sheep in Romania, and livestock in Argentina intended for the Entente forces.^[91] One of these German saboteurs was Anton Dilger. Also, Germany itself became a victim of similar attacks – horses bound for Germany were infected with Burkholderia by French operatives in Switzerland.^[92]

During World War II, the U.S. and Canada secretly investigated the use of rinderpest, a highly lethal disease of cattle, as a bioweapon.^[91][93]

In the 1980s Soviet Ministry of Agriculture had successfully developed variants of foot-and-mouth disease, and rinderpest against cows, African swine fever for pigs, and psittacosis to kill the chicken. These agents were prepared to spray them down from tanks attached to airplanes over hundreds of miles. The secret program was code-named "Ecology".^[55]

During the Mau Mau Uprising in 1952, the poisonous latex of the African milk bush was used to kill cattle.^[94]

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Biological hazard

Biological hazard

A biological hazard, or biohazard, is a biological substance that poses a threat to the health of living organisms, primarily humans. This could include a sample of a microorganism, virus or toxin that can adversely affect human health. A biohazard could also be a substance harmful to other living beings.

Infectivity

Infectivity

In epidemiology, infectivity is the ability of a pathogen to establish an infection. More specifically, infectivity is a pathogen's capacity for horizontal transmission — that is, how frequently it spreads among hosts that are not in a parent–child relationship. The measure of infectivity in a population is called incidence.

Bacillus anthracis

Bacillus anthracis

Bacillus anthracis is a gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease to livestock and, occasionally, to humans. It is the only permanent (obligate) pathogen within the genus Bacillus. Its infection is a type of zoonosis, as it is transmitted from animals to humans. It was discovered by a German physician Robert Koch in 1876, and became the first bacterium to be experimentally shown as a pathogen. The discovery was also the first scientific evidence for the germ theory of diseases.

Influenza

Influenza

Influenza, commonly known as "the flu", is an infectious disease caused by influenza viruses. Symptoms range from mild to severe and often include fever, runny nose, sore throat, muscle pain, headache, coughing, and fatigue. These symptoms begin from one to four days after exposure to the virus and last for about 2–8 days. Diarrhea and vomiting can occur, particularly in children. Influenza may progress to pneumonia, which can be caused by the virus or by a subsequent bacterial infection. Other complications of infection include acute respiratory distress syndrome, meningitis, encephalitis, and worsening of pre-existing health problems such as asthma and cardiovascular disease.

Mediastinitis

Mediastinitis

Mediastinitis is inflammation of the tissues in the mid-chest, or mediastinum. It can be either acute or chronic. It is thought to be due to four different etiologies:direct contamination hematogenous or lymphatic spread extension of infection from the neck or retroperitoneum extension from the lung or pleura

Antibiotic

Antibiotic

An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting bacterial infections, and antibiotic medications are widely used in the treatment and prevention of such infections. They may either kill or inhibit the growth of bacteria. A limited number of antibiotics also possess antiprotozoal activity. Antibiotics are not effective against viruses such as the common cold or influenza; drugs which inhibit growth of viruses are termed antiviral drugs or antivirals rather than antibiotics. They are also not effective against fungi; drugs which inhibit growth of fungi are called antifungal drugs.

Brucella

Brucella

Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855–1931). They are small, nonencapsulated, nonmotile, facultatively intracellular coccobacilli.

Burkholderia mallei

Burkholderia mallei

Burkholderia mallei is a Gram-negative, bipolar, aerobic bacterium, a human and animal pathogen of genus Burkholderia causing glanders; the Latin name of this disease (malleus) gave its name to the species causing it. It is closely related to B. pseudomallei, and by multilocus sequence typing it is a subspecies of B. pseudomallei. B. mallei evolved from B. pseudomallei by selective reduction and deletions from the B. pseudomallei genome. Unlike B. pseudomallei and other genus members, B. mallei is nonmotile; its shape is coccobacillary measuring some 1.5–3.0 μm in length and 0.5–1.0 μm in diameter with rounded ends.

Burkholderia pseudomallei

Burkholderia pseudomallei

Burkholderia pseudomallei is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It was reported in 2008 that there had been an expansion of the affected regions due to significant natural disasters, and it could be found in Southern China, Hong Kong, and countries in America. B. pseudomallei, amongst other pathogens, has been found in monkeys imported into the United States from Asia for laboratory use, posing a risk that the pathogen could be introduced into the country.

Coxiella burnetii

Coxiella burnetii

Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. C. burnetii is a small Gram-negative, coccobacillary bacterium that is highly resistant to environmental stresses such as high temperature, osmotic pressure, and ultraviolet light. These characteristics are attributed to a small cell variant form of the organism that is part of a biphasic developmental cycle, including a more metabolically and replicatively active large cell variant form. It can survive standard disinfectants, and is resistant to many other environmental changes like those presented in the phagolysosome.

Francisella tularensis

Francisella tularensis

Francisella tularensis is a pathogenic species of Gram-negative coccobacillus, an aerobic bacterium. It is nonspore-forming, nonmotile, and the causative agent of tularemia, the pneumonic form of which is often lethal without treatment. It is a fastidious, facultative intracellular bacterium, which requires cysteine for growth. Due to its low infectious dose, ease of spread by aerosol, and high virulence, F. tularensis is classified as a Tier 1 Select Agent by the U.S. government, along with other potential agents of bioterrorism such as Yersinia pestis, Bacillus anthracis, and Ebola virus. When found in nature, Francisella tularensis can survive for several weeks at low temperatures in animal carcasses, soil, and water. In the laboratory, F. tularensis appears as small rods, and is grown best at 35–37 °C.

Rickettsia prowazekii

Rickettsia prowazekii

Rickettsia prowazekii is a species of gram-negative, alphaproteobacteria, obligate intracellular parasitic, aerobic bacillus bacteria that is the etiologic agent of epidemic typhus, transmitted in the feces of lice. In North America, the main reservoir for R. prowazekii is the flying squirrel. R. prowazekii is often surrounded by a protein microcapsular layer and slime layer; the natural life cycle of the bacterium generally involves a vertebrate and an invertebrate host, usually an arthropod, typically the human body louse. A form of R. prowazekii that exists in the feces of arthropods remains stably infective for months. R. prowazekii also appears to be the closest free-living relative of mitochondria, based on genome sequencing.

Medical countermeasures

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In 2010 at The Meeting of the States Parties to the Convention on the Prohibition of the Development, Production and Stockpiling of Bacteriological (Biological) and Toxin Weapons and Their Destruction in Geneva^[95] the sanitary epidemiological reconnaissance was suggested as well-tested means for enhancing the monitoring of infections and parasitic agents, for the practical implementation of the International Health Regulations (2005). The aim was to prevent and minimize the consequences of natural outbreaks of dangerous infectious diseases as well as the threat of alleged use of biological weapons against BTWC States Parties.

Many countries require their active-duty military personnel to get vaccinated for certain diseases that may potentially be used as a bioweapon such as anthrax.^[96]

Public health and disease surveillance

It is important to note that most classical and modern biological weapons' pathogens can be obtained from a plant or an animal which is naturally infected.^[97]

In the largest biological weapons accident known—the anthrax outbreak in Sverdlovsk (now Yekaterinburg) in the Soviet Union in 1979—sheep became ill with anthrax as far as 200 kilometers from the release point of the organism from a military facility in the southeastern portion of the city and still off-limits to visitors today, (see Sverdlovsk Anthrax leak).^[98]

Thus, a robust surveillance system involving human clinicians and veterinarians may identify a bioweapons attack early in the course of an epidemic, permitting the prophylaxis of disease in the vast majority of people (and/or animals) exposed but not yet ill.^[99]

For example, in the case of anthrax, it is likely that by 24–36 hours after an attack, some small percentage of individuals (those with the compromised immune system or who had received a large dose of the organism due to proximity to the release point) will become ill with classical symptoms and signs (including a virtually unique chest X-ray finding, often recognized by public health officials if they receive timely reports).^[100] The incubation period for humans is estimated to be about 11.8 days to 12.1 days. This suggested period is the first model that is independently consistent with data from the largest known human outbreak. These projections refine previous estimates of the distribution of early-onset cases after a release and support a recommended 60-day course of prophylactic antibiotic treatment for individuals exposed to low doses of anthrax.^[101] By making these data available to local public health officials in real time, most models of anthrax epidemics indicate that more than 80% of an exposed population can receive antibiotic treatment before becoming symptomatic, and thus avoid the moderately high mortality of the disease.^[100]

Common epidemiological warnings

From most specific to least specific:^[102]

1. Single cause of a certain disease caused by an uncommon agent, with lack of an epidemiological explanation.
2. Unusual, rare, genetically engineered strain of an agent.
3. High morbidity and mortality rates in regards to patients with the same or similar symptoms.
4. Unusual presentation of the disease.
5. Unusual geographic or seasonal distribution.
6. Stable endemic disease, but with an unexplained increase in relevance.
7. Rare transmission (aerosols, food, water).
8. No illness presented in people who were/are not exposed to "common ventilation systems (have separate closed ventilation systems) when illness is seen in persons in close proximity who have a common ventilation system."
9. Different and unexplained diseases coexisting in the same patient without any other explanation.
10. Rare illness that affects a large, disparate population (respiratory disease might suggest the pathogen or agent was inhaled).
11. Illness is unusual for a certain population or age-group in which it takes presence.
12. Unusual trends of death and/or illness in animal populations, previous to or accompanying illness in humans.
13. Many affected reaching out for treatment at the same time.
14. Similar genetic makeup of agents in affected individuals.
15. Simultaneous collections of similar illness in non-contiguous areas, domestic, or foreign.
16. An abundance of cases of unexplained diseases and deaths.

Bioweapon identification

The goal of biodefense is to integrate the sustained efforts of the national and homeland security, medical, public health, intelligence, diplomatic, and law enforcement communities. Health care providers and public health officers are among the first lines of defense. In some countries private, local, and provincial (state) capabilities are being augmented by and coordinated with federal assets, to provide layered defenses against biological weapon attacks. During the first Gulf War the United Nations activated a biological and chemical response team, Task Force Scorpio, to respond to any potential use of weapons of mass destruction on civilians.

The traditional approach toward protecting agriculture, food, and water: focusing on the natural or unintentional introduction of a disease is being strengthened by focused efforts to address current and anticipated future biological weapons threats that may be deliberate, multiple, and repetitive.

The growing threat of biowarfare agents and bioterrorism has led to the development of specific field tools that perform on-the-spot analysis and identification of encountered suspect materials. One such technology, being developed by researchers from the Lawrence Livermore National Laboratory (LLNL), employs a "sandwich immunoassay", in which fluorescent dye-labeled antibodies aimed at specific pathogens are attached to silver and gold nanowires.^[103]

In the Netherlands, the company TNO has designed Bioaerosol Single Particle Recognition eQuipment (BiosparQ). This system would be implemented into the national response plan for bioweapon attacks in the Netherlands.^[104]

Researchers at Ben Gurion University in Israel are developing a different device called the BioPen, essentially a "Lab-in-a-Pen", which can detect known biological agents in under 20 minutes using an adaptation of the ELISA, a similar widely employed immunological technique, that in this case incorporates fiber optics.^[105]

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Biodefense

Biodefense

Biodefense refers to measures to restore biosecurity to a group of organisms who are, or may be, subject to biological threats or infectious diseases. Biodefense is frequently discussed in the context of biowar or bioterrorism, and is generally considered a military or emergency response term.

Geneva

Geneva

Geneva is the second-most populous city in Switzerland and the most populous city of Romandy, the French-speaking part of Switzerland. Situated in the south west of the country, where the Rhône exits Lake Geneva, it is the capital of the Republic and Canton of Geneva.

Sanitary epidemiological reconnaissance

Sanitary epidemiological reconnaissance

Sanitary epidemiological reconnaissance, synonym epidemiological reconnaissance is a literal name of a concept and routine of finding out disease potential on a territory of arrival of major contingent. Russian: санитарно-эпидемиологическая разведка, син. эпидемиологическая разведка. This is a kind of medical reconnaissance, process of information gathering on possible infectious diseases' origin-sources, the ways and factors of the infection transfer and determining all conditions that could have promoted the spread of infestation among army service personnel. In 1939 Academician E.N.Pavlovsky announced his "doctrine of nidality", so called by Soviet biologists. People can acquire zoonoses and insect-borne diseases when they occupy at certain times of the year natural habitat of a certain pathogen. The WHO Expert Committee on Zoonoses listed over 100 such diseases. About natural focality of the diseases is known elsewhere.

International Health Regulations

International Health Regulations

The International Health Regulations (IHR), first adopted by the World Health Assembly in 1969 and last revised in 2005, are a legally binding rules that only apply to the WHO that is an instrument that aims for international collaboration "to prevent, protect against, control, and provide a public health response to the international spread of disease in ways that are commensurate with and restricted to public health risks and that avoid unnecessary interference with international traffic and trade". The IHR is the only international legal treaty with the responsibility of empowering the World Health Organization (WHO) to act as the main global surveillance system.

Military

Military

A military, also known collectively as armed forces, is a heavily armed, highly organized force primarily intended for warfare. It is typically authorized and maintained by a sovereign state, with its members identifiable by their distinct military uniform. It may consist of one or more military branches such as an army, navy, air force, space force, marines, or coast guard. The main task of the military is usually defined as defence of the state and its interests against external armed threats.

Soviet Union

Soviet Union

The Soviet Union, officially the Union of Soviet Socialist Republics (USSR), was a transcontinental country that spanned much of Eurasia from 1922 to 1991. A flagship communist state, it was nominally a federal union of fifteen national republics; in practice, both its government and its economy were highly centralized until its final years. It was a one-party state governed by the Communist Party of the Soviet Union, with the city of Moscow serving as its capital as well as that of its largest and most populous republic: the Russian SFSR. Other major cities included Leningrad, Kiev, Minsk, Tashkent, Alma-Ata, and Novosibirsk. It was the largest country in the world, covering over 22,402,200 square kilometres (8,649,500 sq mi) and spanning eleven time zones.

Task Force Scorpio

Task Force Scorpio

Task Force Scorpio was a United Nations biological and chemical weapons response team that was activated during the first Gulf War to respond to any reported use of weapons of mass destruction on civilians. The task force was composed of volunteer Swiss members of the Disaster Relief Unit and international specialists, equipped with the latest chemical and biological protection equipment, immunized against agents suspected for battlefield use and had its own jet transportation. The members of the team were to travel under Swiss diplomatic passports anywhere in the world in less than 24 hours of need.

Lawrence Livermore National Laboratory

Lawrence Livermore National Laboratory

Lawrence Livermore National Laboratory (LLNL) is a federally funded research and development center in Livermore, California, United States. Originally established in 1952, the laboratory now is sponsored by the United States Department of Energy and administrated by Lawrence Livermore National Security, LLC.

Pathogen

Pathogen

In biology, a pathogen in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a germ.

Netherlands

Netherlands

The Netherlands, informally Holland, is a country located in northwestern Europe with overseas territories in the Caribbean. It is the largest of four constituent countries of the Kingdom of the Netherlands. The Netherlands consists of twelve provinces; it borders Germany to the east, and Belgium to the south, with a North Sea coastline to the north and west. It shares maritime borders with the United Kingdom, Germany and Belgium in the North Sea. The country's official language is Dutch, with West Frisian as a secondary official language in the province of Friesland. Dutch, English and Papiamento are official in the Caribbean territories.

Netherlands Organisation for Applied Scientific Research

Netherlands Organisation for Applied Scientific Research

Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek is an independent research organisation in the Netherlands that focuses on applied science.

ELISA

ELISA

The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.

United States

• Fort Detrick, Maryland
  

  Researchers working in Class III cabinets at the U.S. Army Biological Warfare Laboratories, Camp Detrick, Maryland (1940s).
  • U.S. Army Biological Warfare Laboratories (1943–69)
    • Building 470
    • One-Million-Liter Test Sphere
    • Operation Sea-Spray
    • Operation Whitecoat (1954–73)
  • U.S. entomological warfare program
    • Operation Big Itch
    • Operation Big Buzz
    • Operation Drop Kick
    • Operation May Day
• Project Bacchus
• Project Clear Vision
• Project SHAD
• Project 112
• Horn Island Testing Station
• Fort Terry
• Granite Peak Installation
• Vigo Ordnance Plant

United Kingdom

• Porton Down
• Gruinard Island
• Nancekuke
• Operation Vegetarian (1942–1944)
• Open-air field tests:
  • Operation Harness off Antigua, 1948–1950.
  • Operation Cauldron off Stornoway, 1952.
  • Operation Hesperus off Stornoway, 1953.
  • Operation Ozone off Nassau, 1954.
  • Operation Negation off Nassau, 1954–5.

Soviet Union and Russia

• Biopreparat (18 labs and production centers)
  • Stepnogorsk Scientific and Technical Institute for Microbiology, Stepnogorsk, northern Kazakhstan
  • Institute of Ultra Pure Biochemical Preparations, Leningrad, a weaponized plague center
  • Vector State Research Center of Virology and Biotechnology (VECTOR), a weaponized smallpox center
  • Institute of Applied Biochemistry, Omutninsk
  • Kirov bioweapons production facility, Kirov, Kirov Oblast
  • Zagorsk smallpox production facility, Zagorsk
  • Berdsk bioweapons production facility, Berdsk
  • Bioweapons research facility, Obolensk
  • Sverdlovsk bioweapons production facility (Military Compound 19), Sverdlovsk, a weaponized anthrax center
• Institute of Virus Preparations
• Poison laboratory of the Soviet secret services
• Vozrozhdeniya
• Project Bonfire
• Project Factor

Japan

U.S. authorities granted Unit 731 officials immunity from prosecution in return for access to their research.

• Unit 731
• Zhongma Fortress
• Kaimingjie germ weapon attack
• Khabarovsk War Crime Trials
• Epidemic Prevention and Water Purification Department

Iraq

• Al Hakum
• Salman Pak facility
• Al Manal facility

South Africa

• Project Coast
• Delta G Scientific Company
• Roodeplaat Research Laboratories
• Protechnik

Rhodesia

Canada

• Grosse Isle, Quebec, site (1939–45) of research into anthrax and other agents
• DRDC Suffield, Suffield, Alberta

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Fort Detrick

Fort Detrick

Fort Detrick is a United States Army Futures Command installation located in Frederick, Maryland. Fort Detrick was the center of the U.S. biological weapons program from 1943 to 1969. Since the discontinuation of that program, it has hosted most elements of the United States biological defense program.

Biosafety level

Biosafety level

A biosafety level (BSL), or pathogen/protection level, is a set of biocontainment precautions required to isolate dangerous biological agents in an enclosed laboratory facility. The levels of containment range from the lowest biosafety level 1 (BSL-1) to the highest at level 4 (BSL-4). In the United States, the Centers for Disease Control and Prevention (CDC) have specified these levels. In the European Union, the same biosafety levels are defined in a directive. In Canada the four levels are known as Containment Levels. Facilities with these designations are also sometimes given as P1 through P4, as in the term P3 laboratory.

Maryland

Maryland

Maryland is a state in the Mid-Atlantic region of the United States. It borders Virginia, West Virginia, and the District of Columbia to its south and west; Pennsylvania to its north; and Delaware and the Atlantic Ocean to its east. With a total land area of 12,407 square miles (32,130 km2), Maryland is the 8th smallest state by land area, but with a population of over 6,177,200, it ranks as the 18th most populous state and the 5th most densely populated. Baltimore is the largest city in the state, and the capital is Annapolis. Among its occasional nicknames are Old Line State, the Free State, and the Chesapeake Bay State. It is named after Henrietta Maria, the French-born queen of England, Scotland, and Ireland, who was known then in England as Mary.

Building 470

Building 470

Building 470 — also called the Pilot Plant, or sometimes “the Tower”, or “Anthrax Tower” — was a seven-story steel and brick building at Fort Detrick in Frederick, Maryland, United States, used in the small-scale production of biological warfare (BW) agents. The building, a Cold War era structure, was transferred from the Department of Defense to the National Cancer Institute-Frederick in 1988, to which it belonged until 2003 when it was demolished.

One-Million-Liter Test Sphere

One-Million-Liter Test Sphere

The One-Million-Liter Test Sphere—also known as the Test Sphere, the Horton Test Sphere, the Cloud Study Chamber, Building 527, and the “Eight Ball” —is a decommissioned biological warfare (BW) chamber and testing facility located on Fort Detrick, Maryland, USA. It was constructed and utilized by the U.S. Army Biological Warfare Laboratories as part of its BW research program from 1951 to 1969. It is the largest aerobiology chamber ever constructed and was placed on the National Register of Historic Places in 1977.

Operation Sea-Spray

Operation Sea-Spray

Operation Sea-Spray was a 1950 U.S. Navy secret biological warfare experiment in which Serratia marcescens and Bacillus globigii bacteria were sprayed over the San Francisco Bay Area in California, in order to determine how vulnerable a city like San Francisco may be to a bioweapon attack.

Operation Whitecoat

Operation Whitecoat

Operation Whitecoat was a biodefense medical research program carried out by the United States Army at Fort Detrick, Maryland between 1954 and 1973. The program pursued medical research using volunteer enlisted personnel who were eventually nicknamed "Whitecoats". These volunteers, all conscientious objectors, including many members of the Seventh-day Adventist Church, were informed of the purpose and goals of each project before providing consent to participate in any project. The stated purpose of the research was to defend troops and civilians against biological weapons.

Operation Big Itch

Operation Big Itch

Operation Big Itch was a U.S. entomological warfare field test using uninfected fleas to determine their coverage and survivability as a vector for biological agents. The tests were conducted at Dugway Proving Ground in 1954.

Operation Big Buzz

Operation Big Buzz

Operation Big Buzz was a U.S. military entomological warfare field test conducted in the U.S. state of Georgia in 1955. The tests involved dispersing over 300,000 mosquitoes from aircraft and through ground dispersal methods.

Operation Drop Kick

Operation Drop Kick

Operation Drop Kick was conducted between April and November 1956 by the US Army Chemical Corps to test the practicality of employing mosquitoes to carry an entomological warfare agent in different ways. The Chemical Corps released uninfected female mosquitoes into a cooperative residential area of Savannah, Georgia, and then estimated how many mosquitoes entered houses and bit people. Within a day, the mosquitoes had bitten many people. In 1958, the Chemical Corps released 1,000,000 mosquitoes in Avon Park, Florida.

Operation May Day

Operation May Day

Operation May Day was a series of entomological warfare (EW) tests conducted by the U.S. military in Savannah, Georgia in 1956.

Project 112

Project 112

Project 112 was a biological and chemical weapon experimentation project conducted by the United States Department of Defense from 1962 to 1973.

Bioweaponeers:

Includes scientists and administrators

Writers and activists:

Discover more about List of associated people related topics

Ira Baldwin

Ira Baldwin

Ira Lawrence Baldwin was the founder and director emeritus of the Wisconsin Academy Foundation. He began teaching bacteriology at the University of Wisconsin in 1927 and a few years later moved into what became a career in administration. He held positions as chair of the Department of Bacteriology, dean of the Graduate School, dean and director of the College of Agriculture, university vice president for academic affairs, and special assistant to the president. He was also involved in programs for agricultural development both in the United States and abroad. Ira Baldwin wrote a hostile review of Rachel Carson's Silent Spring, titled "Chemicals and Pests," in the journal Science.

Kurt Blome

Kurt Blome

Kurt Blome was a high-ranking Nazi scientist before and during World War II. He was the Deputy Reich Health Leader (Reichsgesundheitsführer) and Plenipotentiary for Cancer Research in the Reich Research Council. In his autobiography Arzt im Kampf, he equated medical and military power in their battle for life and death.

Anton Dilger

Anton Dilger

Anton Casimir Dilger was a German-American medical doctor and a main actor in the German biological warfare sabotage program during World War I. His father, Hubert Dilger, was a United States Army captain who had received the Medal of Honor for his work as an artilleryman at the Battle of Chancellorsville (1863) during the American Civil War. Dilger eventually fled to Madrid, Spain, where he died during the 1918 flu pandemic.

Paul Fildes

Paul Fildes

Sir Paul Gordon Fildes was a British pathologist and microbiologist who worked on the development of chemical-biological weaponry at Porton Down during the Second World War.

Arthur Galston

Arthur Galston

Arthur W. Galston was an American plant physiologist and bioethicist. As a plant biologist, Galston studied plant hormones and the effects of light on plant development, particularly phototropism. He identified riboflavin and other flavins as the photoreceptors for phototropism, the bending of plants toward light, challenging the prevailing view that carotenoids were responsible.

Riley D. Housewright

Riley D. Housewright

Riley D. Housewright was an American microbiologist who conducted research on germ warfare. Having been assigned to the Fort Detrick laboratory in the mid-1940s and appointed scientific director in 1956, Housewright played a major role in the development of bioweapons for a proposed attack on Cuba during the Cuban Missile Crisis. In 1966, he was appointed president of the American Society for Microbiology. He left Fort Detrick in 1970 following President Nixon's moratorium on US bioweapons research, and later published several books on water safety for the National Academy of Sciences. In the 1980s, Housewright became executive director of the American Society for Microbiology. He died in Frederick, Maryland, in 2003.

Elvin A. Kabat

Elvin A. Kabat

Elvin Abraham Kabat was an American biomedical scientist and one of the founding fathers of quantitative immunochemistry. Kabat was awarded the Louisa Gross Horwitz Prize from Columbia University in 1977, National Medal of Science in 1991, and American Association of Immunologists Lifetime Achievement Award in 1995. He is the father of Jon Kabat-Zinn.

George W. Merck

George W. Merck

George Wilhelm Herman Emanuel Merck was the president of Merck & Co. from 1925 to 1950 and a member of the Merck family.

Frank Olson

Frank Olson

Frank Rudolph Emmanuel Olson was an American bacteriologist, biological warfare scientist, and an employee of the United States Army Biological Warfare Laboratories (USBWL) who worked at Camp Detrick in Maryland. At a meeting in rural Maryland, he was covertly dosed with LSD by his colleague Sidney Gottlieb and, nine days later, plunged to his death from the window of the Hotel Statler in New York. The U.S. government first described his death as a suicide, and then as misadventure, while others allege murder. The Rockefeller Commission report on the CIA in 1975 acknowledged their having conducted covert drug studies on fellow agents. Olson's death is one of the most mysterious outcomes of the CIA mind control project MKUltra.

Prince Tsuneyoshi Takeda

Prince Tsuneyoshi Takeda

Prince Tsuneyoshi Takeda was the second and last heir of the Takeda-no-miya collateral branch of the Japanese Imperial Family.

Huda Salih Mahdi Ammash

Huda Salih Mahdi Ammash

Huda Salih Mahdi Ammash is an Iraqi scientist and academic, often demonized as "Mrs. Anthrax" by Anglo-American media for her unproven association with the discontinued Iraqi biological weapons program as a part of the propaganda campaign to justify the Iraq War. Ammash was number 53 on the Pentagon's list of the 55 most wanted, the five of hearts in the deck of most wanted Iraqi playing cards, and the only woman to be featured. She was captured by coalition forces but later released without being charged.

Erich Traub

Erich Traub

Erich Traub was a German veterinarian, scientist and virologist who specialized in foot-and-mouth disease, Rinderpest and Newcastle disease. Traub was a member of the National Socialist Motor Corps (NSKK), a Nazi motorist corps, from 1938 to 1942. He worked directly for Heinrich Himmler, head of the Schutzstaffel (SS), as the lab chief of the Nazis' leading bio-weapons facility on Riems Island.